Metabolic activation of 1,2‐dibromoethane to a free radical intermediate by rat liver microsomes and isolated hepatocytes

FEBS Letters

Albano, E.³  Vannini, V.¹  Dianzani, M.U.³  Slater, T.F.²  Tomasi, A.² 

[1] Istituto di Patologia Generale, Via Campi 287, 41100 Modena, Italy;Biochemistry Department, Brunel University, Uxbridge, UB8 3PH, England;Istituto di Patologia Generale, Corso Raffaello 30, 10125 Torino Italy

关键词: 1;    2-Dibromoethane;    Free radical;    Spin trapping;    Hypoxia;    Reductive metabolism;    Cytochrome P450;   

DOI  :  10.1016/0014-5793(83)80964-8

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

A one-electron reductive metabolism of 1,2-dibromoethane (DBE) is described that gives rise to a free radical intermediate, which can be stabilized by a spin trapping agent and detected by electron spin resonance spectroscopy. Using rat liver microsomes or isolated hepatocytes from phenobarbitone pretreated animals, under hypoxic conditions, it has been possible to trap a free radical intermediate and identify it by using ¹³C-DBE. Inhibition experiments have demonstrated that the site of activation is the microsomal drug metabolizing system.

【 授权许可】

Unknown