Free radical activation of acetaldehyde and its role in protein alkylation

FEBS Letters

Clot, Paolo³  Albanoa, Emanuele¹  Dianzani, Mario U.³  Tomasi, Aldo²  Comoglio, Adriana³ 

[1] Departments of Medical Sciences, University of Turin, Novara, Italy;Institute of General Pathology, University of Modena, Modena, Italy;Experimental Medicine and Oncology, University of Turin, Novara, Italy

关键词: Acetaldehyde;    Free radical;    Spin trapping;    Xanthine oxidase;   

DOI  :  10.1016/0014-5793(94)00549-4

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The formation of carbon centered free radicals, identified as methylcarbonyl species, was observed using ESR spectroscopy and the spin trapping agent 4-pyridyl-1-oxide-N-t-butyl nitrone (4-POBN) during the oxidation of acetaldehyde by xanthine oxidase. The reaction was dependent upon the presence of OH^• radicals and was inhibited by the addition of superoxide dismutase, catalase or OH^• radical scavengers. The generation of methylcarbonyl radicals was associated with a doubling of stable acetaldehyde adducts with serum albumin, and 4-POBN or superoxide dismutase and catalase, completely blocked this effect. Thus, methylcarbonyl radicals contributed to acetaldehyde-mediated protein alkylation which is involved in causing toxic as well as immunological reactions ascribed to acetaldehyde.

【 授权许可】

Unknown