Pharmacology of Ca2+ release from red beet microsomes suggests the presence of ryanodine receptor homologs in higher plants

FEBS Letters

Sanders, Dale¹  Muir, Shelagh R.¹ 

[1]The Plant Laboratory, University of York, PO Box 373, York Y01 5YW, UK

关键词: Calcium;    Cyclic ADP-ribose;    Ryanodine;    Microsome;    Beta vulgaris;    cADPR;    cyclic ADP-ribose;    InsP3;    inositol 1;    4;    5-trisphosphate;    Mes;    2-[N-morpholino]ethanesulphonic acid;    PMSF;    phenylmethylsulphonyl fluoride;    FCCP;    carbonyl cyanide p-trifluoromethoxyphenylhydrazone;   

DOI  :  10.1016/0014-5793(96)01000-9

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Cyclic ADP-ribose (cADPR) is known to release Ca²⁺ from plant vacuoles, implying that this NAD⁺ metabolite may possess a second messenger role in plants. The degree to which the plant cADPR-gated Ca²⁺ release mechanism resembles cADPR action in animals has been evaluated. cADPR-elicited Ca²⁺ release from red beet microsomes was inhibited by 1 mM procaine but insensitive to heparin. Furthermore, pre-release of Ca²⁺ from red beet vesicles by either 5 mM caffeine or micromolar levels of ryanodine precluded further Ca²⁺ mobilisation by cADPR. Thus, this study argues strongly for conservation between the plant and animal cADPR-elicited Ca²⁺ release mechanisms.

【 授权许可】

Unknown