The three‐dimensional structure of capsule‐specific CMP: 2‐keto‐3‐deoxy‐manno‐octonic acid synthetase from Escherichia coli

FEBS Letters

Jelakovic, Stefan¹  Schulz, Georg E.¹  Jann, Klaus² 

[1] Institut für Organische Chemie und Biochemie, Albert-Ludwigs-Universität, Albertstr. 21, 79104 Freiburg im Breisgau, Germany;Max-Planck-Institut für Immunbiologie, Stübeweg 51, 79108 Freiburg im Breisgau, Germany

关键词: Capsular polysaccharide;    CMP-Kdo synthetase (Escherichia coli);    X-ray analysis;    Crystal structure;    Saccharide activation;    CMP;    cytidine 5′-monophosphate;    CKS;    CMP-Kdo synthetase;    DTT;    dithiothreitol;    K-CKS;    capsule-specific CKS;    Kdo;    2-keto-3-deoxy-manno-octonic acid;    PEG;    polyethylene glycol;    MIR;    multiple isomorphous replacement;    NCS;    non-crystallographic symmetry;   

DOI  :  10.1016/0014-5793(96)00724-7

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

CMP-Kdo synthetases from Gram-negative bacteria activate Kdo for incorporation into lipo- and capsule-polysaccharides. Here we report the crystal structure of the capsule-specific synthetase from E. coli at 2.3 Å resolution. The enzyme is a dimer of 2 × 245 amino acid residues assuming C₂ symmetry. It contains a central predominantly parallel β-sheet with surrounding helices. The chain fold is novel; it is remotely related to a double Rossmann fold. A large pocket at the carboxyl terminal ends of the central β-strands most likely accommodates the catalytic center. A putative phosphate binding site at the loop between the first β-strand and the following helix is indicated by a bound iridium hexachloride anion.

【 授权许可】

Unknown