FEBS Letters | |
The three‐dimensional structure of capsule‐specific CMP: 2‐keto‐3‐deoxy‐manno‐octonic acid synthetase from Escherichia coli | |
Jelakovic, Stefan1  Schulz, Georg E.1  Jann, Klaus2  | |
[1] Institut für Organische Chemie und Biochemie, Albert-Ludwigs-Universität, Albertstr. 21, 79104 Freiburg im Breisgau, Germany;Max-Planck-Institut für Immunbiologie, Stübeweg 51, 79108 Freiburg im Breisgau, Germany | |
关键词: Capsular polysaccharide; CMP-Kdo synthetase (Escherichia coli); X-ray analysis; Crystal structure; Saccharide activation; CMP; cytidine 5′-monophosphate; CKS; CMP-Kdo synthetase; DTT; dithiothreitol; K-CKS; capsule-specific CKS; Kdo; 2-keto-3-deoxy-manno-octonic acid; PEG; polyethylene glycol; MIR; multiple isomorphous replacement; NCS; non-crystallographic symmetry; | |
DOI : 10.1016/0014-5793(96)00724-7 | |
学科分类:生物化学/生物物理 | |
来源: John Wiley & Sons Ltd. | |
【 摘 要 】
CMP-Kdo synthetases from Gram-negative bacteria activate Kdo for incorporation into lipo- and capsule-polysaccharides. Here we report the crystal structure of the capsule-specific synthetase from E. coli at 2.3 Å resolution. The enzyme is a dimer of 2 × 245 amino acid residues assuming C2 symmetry. It contains a central predominantly parallel β-sheet with surrounding helices. The chain fold is novel; it is remotely related to a double Rossmann fold. A large pocket at the carboxyl terminal ends of the central β-strands most likely accommodates the catalytic center. A putative phosphate binding site at the loop between the first β-strand and the following helix is indicated by a bound iridium hexachloride anion.
【 授权许可】
Unknown
【 预 览 】
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