Pyrosequencing-based analysis reveals a novel capsular gene cluster in a KPC-producing Klebsiella pneumoniae clinical isolate identified in Brazil

BMC Microbiology

Marisa Fabiana Nicolás²  Ana Cristina Gales³  Ana Tereza R Vasconcelos²  Alexandra L Gerber²  Luiz Gonzaga P Almeida²  Luiz Fernando Goda Zuleta²  Marsileni Pelisson¹  Eliana Carolina Vespero¹  Renata Cristina Picão³  Pablo Ivan Pereira Ramos² 

[1] Departamento de Patologia Clínica, Análises Clínicas e Toxicologia, Universidade Estadual de Londrina, Londrina, Brazil;Laboratório Nacional de Computação Científica (LNCC), Petrópolis, Rio de Janeiro, Brazil;Laboratório ALERTA, Divisão de Doenças Infecciosas, Universidade Federal de São Paulo, São Paulo, Brazil

关键词: Monosaccharide biosynthesis pathways;    Molecular serotyping;    KPC-producing K. pneumoniae;    K-antigen;    Capsular polysaccharide;    Capsular gene cluster;   

Others  :  1221791
DOI  :  10.1186/1471-2180-12-173

 received in 2012-02-08, accepted in 2012-05-23,  发布年份 2012

【 摘 要 】

Background

An important virulence factor of Klebsiella pneumoniae is the production of capsular polysaccharide (CPS), a thick mucus layer that allows for evasion of the host's defense and creates a barrier against antibacterial peptides. CPS production is driven mostly by the expression of genes located in a locus called cps, and the resulting structure is used to distinguish between different serotypes (K types). In this study, we report the unique genetic organization of the cps cluster from K. pneumoniae Kp13, a clinical isolate recovered during a large outbreak of nosocomial infections that occurred in a Brazilian teaching hospital.

Results

A pyrosequencing-based approach showed that the cps region of Kp13 (cps_Kp13) is 26.4 kbp in length and contains genes common, although not universal, to other strains, such as the rmlBADC operon that codes for L-rhamnose synthesis. cps_Kp13 also presents some unique features, like the inversion of the wzy gene and a unique repertoire of glycosyltransferases. In silico comparison of cps_Kp13 RFLP pattern with 102 previously published cps PCR-RFLP patterns showed that cps_Kp13 is distinct from the C patterns of all other K serotypes. Furthermore, in vitro serotyping showed only a weak reaction with capsular types K9 and K34. We confirm that K9 cps shares common genes with cps_Kp13 such as the rmlBADC operon, but lacks features like uge and Kp13-specific glycosyltransferases, while K34 capsules contain three of the five sugars that potentially form the Kp13 CPS.

Conclusions

We report the first description of a cps cluster from a Brazilian clinical isolate of a KPC-producing K. pneumoniae. The gathered data including K-serotyping support that Kp13’s K-antigen belongs to a novel capsular serotype. The CPS of Kp13 probably includes L-rhamnose and D-galacturonate in its structure, among other residues. Because genes involved in L-rhamnose biosynthesis are absent in humans, this pathway may represent potential targets for the development of antimicrobial agents. Studying the capsular serotypes of clinical isolates is of great importance for further development of vaccines and/or novel therapeutic agents. The distribution of K-types among multidrug-resistant isolates is unknown, but our findings may encourage scientists to perform K-antigen typing of KPC-producing strains worldwide.

【 授权许可】

   
2012 Ramos et al.; licensee BioMed Central Ltd.

【 图 表 】

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