Role of the small RNA RyhB in the Fur regulon in mediating the capsular polysaccharide biosynthesis and iron acquisition systems in Klebsiella pneumoniae

BMC Microbiology

Ching-Ting Lin²  Yi-Ming Hong²  Ying-Tsong Chen³  Chien-Chen Wu¹  Hwei-Ling Peng¹  Chien-Kuo Wang⁴  Su-Hua Huang⁴ 

[1] Department of Biological Science and Technology, National Chiao Tung University, Hsin Chu 30068, Taiwan;School of Chinese Medicine, China Medical University, Taichung 40402, Taiwan;Institute of Molecular and Genomic Medicine, National Health Research Institutes, Miaoli County 35053, Taiwan;Department of Biotechnology, Asia University, Taichung 41354, Taiwan

关键词: Klebsiella pneumoniae;    Iron acquisition system;    Capsular polysaccharide;    Fur;    RyhB;   

Others  :  1221816
DOI  :  10.1186/1471-2180-12-148

 received in 2012-05-15, accepted in 2012-07-09,  发布年份 2012

【 摘 要 】

Background

The capsular polysaccharide (CPS) and iron acquisition systems are important determinants of Klebsiella pneumoniae infections, and we have previously reported that the ferric uptake repressor (Fur) can play dual role in iron acquisition and CPS biosynthesis. In many bacteria, Fur negatively controls the transcription of the small non-coding RNA RyhB to modulate cellular functions and virulence. However, in K. pneumoniae, the role played by RyhB in the Fur regulon has not been characterised. This study investigated Fur regulation of ryhB transcription and the functional role of RyhB in K. pneumoniae.

Results

Deletion of fur from K. pneumoniae increased the transcription of ryhB; the electric mobility shift assay and the Fur-titration assay revealed that Fur could bind to the promoter region of ryhB, suggesting that Fur directly represses ryhB transcription. Additionally, in a Δfur strain with elevated CPS production, deletion of ryhB obviously reduced CPS production. The following promoter-reporter assay and quantitative real-time PCR of cps genes verified that RyhB activated orf1 and orf16 transcription to elevate CPS production. However, deletion of ryhB did not affect the mRNA levels of rcsA, rmpA, or rmpA2. These results imply that Fur represses the transcription of ryhB to mediate the biosynthesis of CPS, which is independent of RcsA, RmpA, and RmpA2. In addition, the Δfur strain’s high level of serum resistance was attenuated by the deletion of ryhB, indicating that RyhB plays a positive role in protecting the bacterium from serum killing. Finally, deletion of ryhB in Δfur reduced the expression of several genes corresponding to 3 iron acquisition systems in K. pneumoniae, and resulted in reduced siderophore production.

Conclusions

The regulation and functional role of RyhB in K. pneumoniae is characterized in this study. RyhB participates in Fur regulon to modulate the bacterial CPS biosynthesis and iron acquisition systems in K. pneumoniae.

【 授权许可】

   
2012 Huang et al.; licensee BioMed Central Ltd.

【 图 表 】

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