FEBS Letters | |
Cloning of a novel ubiquitin‐conjugating enzyme (E2) gene from the ciliate Paramecium tetraurelia | |
Tokushima, Hideyuki1  Nakaoka, Yasuo1  Okano, Satoshi1  Shimizu, Kikuo2  | |
[1] Department of Biophysical Engineering, Faculty of Engineering Science, Osaka University, 1-3 Machikaneyama, Toyonaka, Osaka 560, Japan;Radioisotope Research Center, Osaka University, 1-1 Machikaneyama, Toyonaka, Osaka 560, Japan | |
关键词: Ubiquitin-conjugating enzyme (E2); cDNA cloning; Nucleotide sequence; Phylogenetic tree; Paramecium tetraurelia; | |
DOI : 10.1016/0014-5793(96)00689-8 | |
学科分类:生物化学/生物物理 | |
来源: John Wiley & Sons Ltd. | |
【 摘 要 】
We isolated a 1.7 kb gene (UbcPl) for a ubiquitinconjugating enzyme from a P. tetraurelia cDNA library and sequenced it. Its deduced polypeptide sequence consists of 425 amino acid residues (48 kDa). The UbcP1 protein contains novel N- and C-terminal extensions in addition to a UBC domain, and within the UBC domain it shares low identity with sequences of other known E2s. A constructed phylogenetic tree suggests that the UbcP1 protein may represent a member of a distinct subfamily of E2s. Southern blot analysis showed that the N-terminal extension of the UbcP1 is conserved in P. multimicronucleatum.
【 授权许可】
Unknown
【 预 览 】
Files | Size | Format | View |
---|---|---|---|
RO201912020303033ZK.pdf | 464KB | download |