Selective control of cytosolic glutathione peroxidase and phospholipid hydroperoxide glutathione peroxidase mRNA stability by selenium supply

FEBS Letters

Arthur, John R.¹  Hesketh, John E.¹  Bermano, Giovanna¹ 

[1]Division of Biochemical Sciences, Rowett Research Institute, Bucksburn, Aberdeen AB21 9SB, UK

关键词: Messenger RNA;    Translation;    Selenium;    Glutathione peroxidase (rat hepatoma cell);    Stability;    3′-Untranslated region;    cCGSH-Px;    cytosolic glutathione peroxidase;    PHGSH-Px;    phospholipid hydroperoxide glutathione peroxidase;   

DOI  :  10.1016/0014-5793(96)00493-0

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Selenium depletion of H4 hepatoma cells reduced cytosolic glutathione peroxidase (cGSH-Px) mRNA abundance but had no effect on phospholipid hydroperoxide glutathione peroxidase (PHGSH-Px) mRNA abundance. Actinomycin D chase experiments showed that selenium depletion had no effect on the stability of PHGSH-Px mRNA but decreased the stability of cGSH-Px mRNA. In Se-replete cells puromycin decreased the stability of both cGSH-Px and PHGSH-Px mRNAs. The results suggest that when selenium supply is limiting PHGSH-Px mRNA translation is maintained more than that of cGSH-Px mRNA, and thus more cGSH-Px mRNA is released from polysomes and degraded.

【 授权许可】

Unknown