Thyroid stimulating hormone and selenium supply interact to regulate selenoenzyme gene expression in thyroid cells (FRTL‐5) in culture

FEBS Letters

Arthur, John R.¹  Hesketh, John E.¹  Bermano, Giovanna¹  Villette, Stéphane¹ 

[1] Rowett Research Institute, Greenburn Rd., Bucksburn, Aberdeen AB21 9SB, UK

关键词: Selenium;    Deiodinase;    Thyroid;    Messenger RNA;    3′ Untranslated region;    Thyroid stimulating hormone;   

DOI  :  10.1016/S0014-5793(98)01280-0

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

In the absence of a sodium selenite supplement, FRTL-5 cells showed a reduced activity of cytosolic glutathione peroxidase (cGSH-Px), a marker of selenium status, indicating the cells were Se-deficient. Se-deficient cells showed a 65% reduction in cGSH-Px mRNA abundance but little change in abundance of either phospholipid hydroperoxide glutathione peroxidase or type 1 deiodinase (IDI) mRNA. In Se-replete cells increased thyroid stimulating hormone (TSH) caused a small decrease in IDI abundance but in Se-deficient cells TSH caused a large increase. The results indicate an interaction between TSH and Se status in the regulation of thyroid selenoenzyme synthesis.

【 授权许可】

Unknown