FEBS Letters | |
Activation of mitogen‐activated protein kinase by protein kinase C isotypes α, β I and γ, but not ϵ | |
Tavaré, Jeremy M.1  Dickens, Martin1  Young, Stephen W.1  | |
[1] Department of Biochemistry, School of Medical Sciences, University of Bristol, Bristol BS8 1TD, UK | |
关键词: MAP kinase; Phorbol ester; Insulin; Protein kinase C; MAP kinase; mitogen-activated protein kinase; Erk; extracellular signal-regulated protein kinase; PMA; phorbol 12-myristate 13-acetate; PKC; protein kinase C; MBP; myelin basic protein; | |
DOI : 10.1016/0014-5793(96)00287-6 | |
学科分类:生物化学/生物物理 | |
来源: John Wiley & Sons Ltd. | |
【 摘 要 】
Treatment of CHO.T cells with either PMA or insulin led to the activation of MAP kinase by ∼3-fold, and p90 rsk by ∼ 4-fold. Over-expression of the α, β I or γ isoforms of protein kinase C caused a substantial enhancement of the effect of PMA on the activation of MAP kinase and p90 rsk , however, the effect of insulin was unchanged. Over-expression of the ϵ isoform of protein kinase C did not alter the effect of either PMA or insulin on the activation of MAP kinase and p90 rsk . The results suggest that protein kinase C isotypes α, β I and γ, but not ϵ, can mediate MAP kinase activation by PMA, and strongly support the hypothesis that protein kinase C isoforms can initiate distinct signalling pathways.
【 授权许可】
Unknown
【 预 览 】
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