Insulin activates myelin basic protein (p42 MAP) kinase by a protein kinase C‐independent pathway in rat adipocytes

FEBS Letters

Farese, Robert V.¹  Yang, YunChung¹ 

[1] J.A. Haley Veterans' Hospital and Departments of Internal Medicine and Biochemistry, University of South Florida College of Medicine, University of South Florida, Tampa, FL 33612, USA

关键词: Insulin;    Adipocyte;    MAP kinase;    Phorbol ester;    Protein kinase C;    Glucose transport;   

DOI  :  10.1016/0014-5793(93)80672-H

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Myelin basic protein kinase (MBPK) activity of rat adipocytes was measured directly or in gels after purification of p42 microtubule-associated protein kinase (MAPK). Insulin and phorbol esters provoked 2- to 3-fold increases in MBPK/MAPK activity within 5–10 min. Whereas phorbol ester effects were blocked by protein kinase C (PKC) depletion or inhibition, insulin effects were fully intact, indicating that insulin activates MBPK/MAPK independently of PKC. In contrast, PKC depletion or inhibition markedly inhibited insulin effects on [³H]2-deoxyglucose uptake, suggesting that this effect requires PKC, rather than a factor within the ras/MAPK cascade.

【 授权许可】

Unknown