FEBS Letters | |
Insulin activates myelin basic protein (p42 MAP) kinase by a protein kinase C‐independent pathway in rat adipocytes | |
Farese, Robert V.1  Yang, YunChung1  | |
[1] J.A. Haley Veterans' Hospital and Departments of Internal Medicine and Biochemistry, University of South Florida College of Medicine, University of South Florida, Tampa, FL 33612, USA | |
关键词: Insulin; Adipocyte; MAP kinase; Phorbol ester; Protein kinase C; Glucose transport; | |
DOI : 10.1016/0014-5793(93)80672-H | |
学科分类:生物化学/生物物理 | |
来源: John Wiley & Sons Ltd. | |
【 摘 要 】
Myelin basic protein kinase (MBPK) activity of rat adipocytes was measured directly or in gels after purification of p42 microtubule-associated protein kinase (MAPK). Insulin and phorbol esters provoked 2- to 3-fold increases in MBPK/MAPK activity within 5–10 min. Whereas phorbol ester effects were blocked by protein kinase C (PKC) depletion or inhibition, insulin effects were fully intact, indicating that insulin activates MBPK/MAPK independently of PKC. In contrast, PKC depletion or inhibition markedly inhibited insulin effects on [3H]2-deoxyglucose uptake, suggesting that this effect requires PKC, rather than a factor within the ras/MAPK cascade.
【 授权许可】
Unknown
【 预 览 】
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