Met‐enkephalin induces cytolytic processes of apoptotic type in K562 human erythroid leukemia cells

FEBS Letters

Karelin, Andrey A.¹  Mirkina, Irina I.¹  Blishchenko, Elena Yu.¹  Mernenko, Olga A.¹ 

[1] Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, GSP-7, ul. Miklukho-Maklaya, 16/10, 117871 Moscow, Russia

关键词: Met-enkephalin;    Cytotoxicity;    Apoptosis;    TNF;    tumour necrosis factor;    NT II;    neurotoxin II from Naja naja oxiana cobra venom;   

DOI  :  10.1016/0014-5793(96)00208-6

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Cytolytic activity of Met-enkephalin, an endogenous opioid peptide, was studied within the 10⁻⁷−10⁻¹⁷ M concentration range in K562 human erythroid leukemia cells. Cytolytic activity was determined by the trypan blue inclusion method after 13, 15 and 18 h of Met-enkephalin co-incubation with target cells. Discrete maxima of cytolytic activity were detected at concentrations of 10⁻⁹−10⁻¹⁰, 10⁻¹³ and 10⁻¹⁵ M. Cytolysis was accompanied by internucleosomal DNA fragmentation which is indicative of the mechanism of cell death being apoptosis.

【 授权许可】

Unknown