TNF‐mediated IL6 gene expression and cytotoxicity are co‐inducible in TNF‐resistant L929 cells

FEBS Letters

Fiers, Walter¹  Vandevoorde, Veronique¹  Haegeman, Guy¹ 

[1] Laboratory of Molecular Biology, Gent University, 9000 Gent, Belgium

关键词: Tumor necrosis factor;    Cytotoxicity;    Interleukin-6 induction;    DEX;    dexamethasone;    IFN;    interferon;    IL;    interleukin;    SDS;    sodium dodecyl sulfate;    SSC;    standard saline citrate;    TNF;    tumor necrosis factor;   

DOI  :  10.1016/0014-5793(92)80449-Q

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Interleukin (IL)-6 gene induction was studied in the murine cell line, L929r2, which is resistant to the cytotoxic action of tumor necrosis factor (TNF). Increasing concentrations of TNF slightly elevated the background levels of IL6 expression as compared to non-induced cells. Under conditions where the resistant cells are sensitive to TNF by combined TNF/IFN-γ treatment, the IL6 levels were strongly induced. This induction could be further enhanced by the addition of lithium chloride, or reduced by inhibitors of cytotoxicity, such as dexamethasone. These results confirm our earlier conclusions regarding the close relationship between TNF-mediated IL6 gene expression and the pathway leading to cytotoxicity.

【 授权许可】

Unknown