Two conserved tryptophan residues of tumor necrosis factor and lymphotoxin are not involved in the biological activity

FEBS Letters

Fiers, Walter²  Van Ostade, Xaveer²  Tavernier, Jan¹ 

[1] Biogent, Jozef Plateaustraat 22, Ghent, Belgium;Laboratory of Molecular Biology, State University of Ghent, K.L. Ledeganckstraat, 35 Belgium

关键词: Tumor necrosis factor;    Site-specific mutagenesis;    Cytotoxicity;   

DOI  :  10.1016/0014-5793(88)80510-6

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Each of the two highly conserved tryptophan residues in hTNF (positions 28 and 114) was converted into phenylalanine by site-directed mutagenesis and the mutant proteins were partially purified. A cytotoxicity assay on mouse L929 cells showed only a slight reduction in biological activity, strongly suggesting that neither of the two amino acids is involved in the active site.

【 授权许可】

Unknown