Immunohistochemical analysis of presenilin‐1 expression in the mouse brain

FEBS Letters

Revah, F.¹  Pradier, L.¹  Blanchard, V.¹  Bonici, B.¹  Czech, C.¹  Imperato, A.¹  Moussaoui, S.¹  Gohin, M.¹ 

[1]Rhône-Poulenc Rorer, Centre de Recherche de Vitry-Alfortville, 13 Quai Jules Guesde, BP14, F-94403 Vitry sur Seine Cedex, France

关键词: Neurodegenerative disease;    Alzheimer's disease;    Presenilin;    Chromosome 14;    Immunohistochemistry;    Mouse brain;    Amyloid;   

DOI  :  10.1016/0014-5793(96)00250-5

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

At least 22 different mutations associated with early-onset familial Alzheimer's disease (AD) in various kindreds have been reported to occur in a recently identified gene on chromosome 14, presenilin 1 (PS-1) (Sherrington et al. (1995) Nature 375, 754–760 [1] and reviewed by Van Broeckhoven (1995) Nat. Genet. 11, 230–231 [2]). In order to study the localization of PS-1 in the brain, we raised a polyclonal antiserum specific to a fragment of the predicted protein sequence of PS-1. PS-1 immunostaining was found intracellularly, in the perikaria of discrete cells, mostly neurons, appearing as thick granules, resembling large-size vesicles. These granules were located in the periphery of cell bodies and extended into dendrites and neurites. PS-1 expression was found to be broadly distributed throughout the mouse brain, not only in structures involved in AD pathology, but also in structures unaltered by this disease.

【 授权许可】

Unknown