Streptozotocin, an inducer of NAD+ decrease, attenuates M‐potassium current inhibition by ATP, bradykinin, angiotensin II, endothelin 1 and acetylcholine in NG108‐15 cells

FEBS Letters

Noda, Mami¹  Hoshi, Naoto¹  Egorova, Alla¹  Higashida, Haruhiro¹ 

[1] Department of Biophysics, Kanazawa University School of Medicine, Takara-machi 13-1, Kanazawa 920, Japan

关键词: K+ current;    Signal transduction;    Second messenger;    NAD+;    Neuroblastoma × glioma hybrid cell;   

DOI  :  10.1016/0014-5793(95)01516-7

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The M-potassium current was inhibited by bath application of 100 μM ATP, 10 nM bradykinin, 100 nM angiotensin II and 100 nM endothelin 1 as well as by 10 μM acetylcholine in an ml-muscarinic acetylcholine receptor-transformed NG108-15 cell line. The inhibition of M-current was attenuated in cells pretreated with 5 mM streptozotocin for 5–15 h and restored by simultaneous incubation with 5 mM nicotinamide. The results suggest that signal transduction from these five different receptors to M channels shares a common pathway which is susceptible to a streptozotocin-induced decrease in cellular NAD⁺ content.

【 授权许可】

Unknown