Hydroxyl radical formation from Cu(II)‐trolox mixtures: insights into the pro‐oxidant properties of α‐tocopherol

FEBS Letters

Milne, Lesley¹  Burkitt, Mark J.¹ 

[1] Division of Biochemical Sciences, The Rowett Research Institute, Greenburn Road, Bucksburn, Aberdeen, AB2 9SB, Scotland, UK

关键词: Copper;    ESR Spin trapping;    Hydroxyl radical;    Low density lipoprotein oxidation;    Tocopherol-mediated peroxidation;    Vitamin E;    BC;    bathocuproinedisulfonic acid;    DMSO;    dimethyl sulphoxide;    EDTA;    ethylenediaminetetraacetic acid;    ESR;    electron spin resonance;    LDL;    low density lipoprotein;    MNP;    2-methyl-2-nitrosopropane;    MOPS;    3-(N-morpholino)-propanesulfonic acid;    PBN;    N-t-butyl-α-phenylnitrone;   

DOI  :  10.1016/0014-5793(95)01481-0

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Although widely recognised as the most important chain-breaking antioxidant of the lipid phase, α-tocopherol has also been reported to exert pro-oxidant activity, particularly during the Cu(II)-stimulated oxidation of low density lipoproteins (LDL). In the present communication, we demonstrate that hydroxyl radicals are generated following the interaction of Cu(II) with the α-tocopherol model compound Trolox, involving the reduction of Cu(II) by Trolox and the subsequent reduction of molecular oxygen by Cu(I). We suggest, therefore, that the hydroxyl radical may be the species responsible for the initiation of fatty acid oxidation during the Cu(II)-stimulated oxidation of hydroperoxide-free LDL.

【 授权许可】

Unknown