Biologically relevant metal ion‐dependent hydroxyl radical generation An update

FEBS Letters

Halliwell, Barry¹  Gutteridge, John M.C.² 

[1] Pulmonary-Critical Care Medicine, U.C. Davis Medical Center, 4301 X Street, Sacramento, CA 95817, USA;Oxygen Chemistry Laboratory, Department of Anesthesia and Intensive Care, Royal Brompton Hospital, National Heart and Lung Institute, Sydney Street, London SW3 6LY, UK

关键词: Iron;    Copper;    Hydroxyl radical;    Fenton reaction;    DNA damage;    Superoxide;    Hydrogen peroxide;    DMPO;    5;    5-dimethyl-1-pyrroline-N-oxide;    DETA-PAC;    diethylenetriaminepentaacetic acid;   

DOI  :  10.1016/0014-5793(92)80911-Y

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Transition metal ions, especially iron, appear to be important mediators of oxidative damage in vivo. Iron(II) reacts with H₂O₂ to give more-reactive radicals. On the basis of ESR spin-trapping data with DMPO, supported by aromatic hydroxylation studies and patterns of DNA base modification, it is concluded that hydroxyl radical (OH^•) is likely to be the major damaging species formed in Fenton Systems under biologically-relevant conditions (which include iron concentrations no higher than the micromolar range). Although reactive oxo-iron species (such as ferryl and perferryl) may also be important, chemical evidence for their formation and identity in biologically relevant Fenton systems is currently lacking. Studies at alkaline pH values show that iron(IV) and iron(V) species are highly oxidizing under those reaction conditions, with a pattern of reactivity different from that of OH^•.

【 授权许可】

Unknown