Molecular characterization of a cDNA encoding functional human deoxyhypusine synthase and chromosomal mapping of the corresponding gene locus

FEBS Letters

Kappel, Barbara¹  Steinkasserer, Alexander¹  Hauber, Joachim¹  Bevec, Dorian¹  Jaksche, Herbert¹  Klier, Hannelore¹  Csonga, Robert¹ 

[1] Sandoz Research Institute, Brunner Strasse 59, A-1235 Vienna, Austria

关键词: Eukaryotic initiation factor 5A;    Hypusine formation;    Post-translational modification;    Chromosomal mapping;    bp;    base pair;    eIF-5A;    eukaryotic initiation factor 5A;    RT;    reverse transcription;    PCR;    polymerase chain reaction;   

DOI  :  10.1016/0014-5793(95)01456-X

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Deoxyhypusine synthase is essentially required for the post-translational formation of hypusine, a modification of a specific lysine residue in eukaryotic initiation factor 5A, which appears to be pivotal for cell proliferation. From a human peripheral blood mononuclear cells cDNA library we isolated two independent sequences encoding biologically active deoxyhypusine synthase. DNA sequence analysis revealed a 369 amino acid protein with a molecular mass of 41.055 kDa. This recombinant deoxyhypusine synthase showed significant catalytic activity in synthesis of deoxyhypusine after in vitro transcription and translation as well as upon expression in Escherichia coli. Using a panel of somatic rodent-human cell hybrids we localized the deoxyhupusine synthase gene to human chromosome 19.

【 授权许可】

Unknown