NAD+ stimulated the spermidine‐dependent hypusine formation on the 18 kDa protein in cytosolic lysates derived from NB‐15 mouse neuroblastoma cells

FEBS Letters

Dou, Qing Ping¹  Chen, Kuang Yu¹ 

[1] Department of Chemistry, Rutgers, The State University of New Jersey, New Brunswick, NJ 08903, USA

关键词: NAD+;    Hypusine formation;    Post-translational modification;    Eukaryotic initiation factor 4D;    (Mouse neuroblastoma cell);   

DOI  :  10.1016/0014-5793(88)81149-9

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

When incubated with cultured mouse neuroblastoma cells under growth stimulatory condition, [³H]putrescine or [³H]spermidine can metabolically label a cellular protein of apparent molecular mass 18 kDa. The labeling, which leads to hypusine formation, is due to a covalent linkage between a lysine residue and the butylamino group derived from spermidine. This reaction can be demonstrated in the cytosolic fractions obtained from cells whose spermidine pool was depleted by prior treatment with α-difluoromethylornithine. In an effort to characterize the enzyme system involved in this unique post-translational modification, we found that NAD⁺ at 0.1 mM stimulated labeling more than 150-fold. Other nucleotides such as NADP⁺, ATP and GTP were ineffective. The fact that NAD⁺ dramatically stimulated labeling of the 18 kDa protein indicated that the enzyme involved in hypusine formation may be an NAD⁺-requiring enzyme.

【 授权许可】

Unknown