FEBS Letters | |
NAD+ stimulated the spermidine‐dependent hypusine formation on the 18 kDa protein in cytosolic lysates derived from NB‐15 mouse neuroblastoma cells | |
Dou, Qing Ping1  Chen, Kuang Yu1  | |
[1] Department of Chemistry, Rutgers, The State University of New Jersey, New Brunswick, NJ 08903, USA | |
关键词: NAD+; Hypusine formation; Post-translational modification; Eukaryotic initiation factor 4D; (Mouse neuroblastoma cell); | |
DOI : 10.1016/0014-5793(88)81149-9 | |
学科分类:生物化学/生物物理 | |
来源: John Wiley & Sons Ltd. | |
【 摘 要 】
When incubated with cultured mouse neuroblastoma cells under growth stimulatory condition, [3H]putrescine or [3H]spermidine can metabolically label a cellular protein of apparent molecular mass 18 kDa. The labeling, which leads to hypusine formation, is due to a covalent linkage between a lysine residue and the butylamino group derived from spermidine. This reaction can be demonstrated in the cytosolic fractions obtained from cells whose spermidine pool was depleted by prior treatment with α-difluoromethylornithine. In an effort to characterize the enzyme system involved in this unique post-translational modification, we found that NAD+ at 0.1 mM stimulated labeling more than 150-fold. Other nucleotides such as NADP+, ATP and GTP were ineffective. The fact that NAD+ dramatically stimulated labeling of the 18 kDa protein indicated that the enzyme involved in hypusine formation may be an NAD+-requiring enzyme.
【 授权许可】
Unknown
【 预 览 】
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