The Escherichia coli cysG gene encodes the multifunctional protein, siroheme synthase

FEBS Letters

Spencer, Jonathan B.¹  Scott, A.Ian¹  Roessner, Charles A.¹  Stolowich, Neal J.¹ 

[1] Center for Biological NMR, Department of Chemistry, Texas A&M University, College Station, TX 77843-3255, USA

关键词: Siroheme;    Dehydrogenase;    Sirohydrochlorin;    Ferrochelatase;    NAD+;   

DOI  :  10.1016/0014-5793(93)80438-Z

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Previously, the E. coli cysG gene product had been shown to sequentially methylate uro'gen III to produce precorrin-2, hence it was given the trivial name uro'gen III methylase. We now report that in addition to methylase activity, the CysG protein catalyses both the NAD⁺ dependent oxidation of precorrin-2 to sirohydrochlorin, but also the insertion of iron into this oxidized intermediate, thereby producing siroheme. Thus CysG is a multifunctional protein solely responsible for siroheme synthesis from uro'gen III in E. coli, and accordingly is renamed siroheme synthase.

【 授权许可】

Unknown