Tissue transglutaminase expression affects hypusine metabolism in BALB/c 3T3 cells

FEBS Letters

Beninati, S¹  Caraglia, M²  Lentini, A¹  Tagliaferri, P³  Abbruzzese, A²  Gentile, V² 

[1] Department of Biology, II University of Rome, Tor Vergata, Rome, Italy;Department of Biochemistry and Biophysics, II University of Naples, Via Costantinopoli, 80132, Naples, Italy;Department of Molecular and Clinical Endocrinology and Oncology, University ‘Federico II’ of Naples, Naples, Italy

关键词: Transglutaminase;    Eukaryotic initiation factor 5A;    Hypusine;    Polyamine;    Post-translational modification;    eIF-5A;    eukaryotic initiation factor 5A;    TGase;    transglutaminase;    EGF;    epidermal growth factor;    TGFβ;    transforming growth factor β;    IL-6;    interleukin-6;    PBS;    phosphate buffered saline;    HPLC;    high performance liquid chromatography;    SDS-PAGE;    sodium-dodecylsulfate polyacrylamide gel electrophoresis;   

DOI  :  10.1016/S0014-5793(98)01191-0

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Post-translational formation of hypusine in eukaryotic initiation factor 5A (eIF-5A) is essential for cell viability. Recently, we showed that hypusine protein is an in vitro substrate for transglutaminases (TGases). We report the effect of tissue TGase expression on the in vivo hypusine metabolic pathway. The stable expression of tTGase in BALB/c 3T3 cells induced a 100-fold reduction of hypusine levels and a 50% increase of γ-glutamyl-ϖ-hypusine formation. Such changes were paralleled by a consistent decrease in the free polyamine pool and an enhancement of their excretion and of the formation of their γ-glutamyl derivatives. These effects occurred together with a significant reduction of cell proliferation. In this report we suggest, for the first time, that tTGase affects hypusine metabolism, thus regulating the eIF-5A activity and cell proliferation.

【 授权许可】

Unknown