cDNA and derived amino acid sequence of the hypusine containing protein from Dictyostelium discoideum

FEBS Letters

Lottspeich, Friedrich¹  Noegel, Angelika A.¹  Sandholzer, Ute¹  Centea-Intemann, Mihaela¹ 

[1] Max-Planck-Institut für Biochemie, D-8033 Martinsried, München, FRG

关键词: Initiation factor eIF-4D;    Posttranslational modification;    Polyamine;    Hypusine;   

DOI  :  10.1016/0014-5793(89)80260-1

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The eukaryotic translation initiation factor eIF-4D is the only protein known to contain the unusual amino acid hypusine, a posttranslationally modified lysine. For the production of monoclonal antibodies the hypusine-containing protein (HP) was isolated from Dictyostelium discoideum. Using these monoclonal antibodies, a full-length cDNA clone was isolated from a λgt11 library. The D. discoideum HP consists of 169 amino acids and has a molecular mass of 18.3 kDa. It is encoded by a single gene. Tryptic and cyanogen bromide peptides were prepared from the purified protein and sequenced. The hypusine residue is located at amino acid position 65 of the HP. The corresponding mRNA of approx. 0.6 kb is present throughout the life cycle of D. discoideum.

【 授权许可】

Unknown