FEBS Letters | |
Deoxyhypusine synthase gene is essential for cell viability in the yeast Saccharomyces cerevisiae | |
Abid, Ruhul1  Sasaki, Kazuhiro1  Miyazaki, Masazumi1  | |
[1] Department of Molecular Biology, School of Science, Nagoya University, Chikusa-ku, Nagoya 464-01, Japan | |
关键词: Deoxyhypusine synthase; Hypusine; Eukaryotic initiation factor 5A; Post-translational modification; Gene disruption; ec-eIF-5A; the precursor of eIF-5A expressed in E. coli from yeast eIF-5A genes; PAGE; polyacrylamide gel electrophoresis; PCR; polymerase chain reaction; IPTG; isopropyl-1-thio-β-d-galacto-pyranoside; PMSF; phenylmethylsulfonyl fluoride; | |
DOI : 10.1016/0014-5793(96)00310-9 | |
学科分类:生物化学/生物物理 | |
来源: John Wiley & Sons Ltd. | |
【 摘 要 】
Deoxyhypusine synthase catalyzes the first of two steps in the biosynthesis of hypusine, a modification of a specific lysine residue in the precursor of eukaryotic translation initiation factor 5A. We have purified deoxyhypusine synthase from yeast, and cloned and sequenced the corresponding gene encoding a 387-amino acid protein from Saccharomyces cerevisiae. Gene disruption experiments indicated that the deoxyhypusine synthase gene is essential for cell growth in yeast. This gene was shown to be an intron-free, single-copy gene, and its product can catalyze the synthesis of deoxyhypusine equally in two precursor forms of eIF-5A, derived from two distinct genes of yeast.
【 授权许可】
Unknown
【 预 览 】
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