Deoxyhypusine synthase gene is essential for cell viability in the yeast Saccharomyces cerevisiae

FEBS Letters

Abid, Ruhul¹  Sasaki, Kazuhiro¹  Miyazaki, Masazumi¹ 

[1] Department of Molecular Biology, School of Science, Nagoya University, Chikusa-ku, Nagoya 464-01, Japan

关键词: Deoxyhypusine synthase;    Hypusine;    Eukaryotic initiation factor 5A;    Post-translational modification;    Gene disruption;    ec-eIF-5A;    the precursor of eIF-5A expressed in E. coli from yeast eIF-5A genes;    PAGE;    polyacrylamide gel electrophoresis;    PCR;    polymerase chain reaction;    IPTG;    isopropyl-1-thio-β-d-galacto-pyranoside;    PMSF;    phenylmethylsulfonyl fluoride;   

DOI  :  10.1016/0014-5793(96)00310-9

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Deoxyhypusine synthase catalyzes the first of two steps in the biosynthesis of hypusine, a modification of a specific lysine residue in the precursor of eukaryotic translation initiation factor 5A. We have purified deoxyhypusine synthase from yeast, and cloned and sequenced the corresponding gene encoding a 387-amino acid protein from Saccharomyces cerevisiae. Gene disruption experiments indicated that the deoxyhypusine synthase gene is essential for cell growth in yeast. This gene was shown to be an intron-free, single-copy gene, and its product can catalyze the synthesis of deoxyhypusine equally in two precursor forms of eIF-5A, derived from two distinct genes of yeast.

【 授权许可】

Unknown