S‐Phase phosphorylation of lamin B2

FEBS Letters

Kill, Ian R.¹  Hutchison, Christopher J.¹ 

[1] Department of Biological Sciences, The University of Dundee, Dundee DD1 4HN, Scotland, UK

关键词: Human fibroblast;    Cell cycle;    Lamin phosphorylation;   

DOI  :  10.1016/0014-5793(95)01302-4

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Lamin B2 modification in synchronously dividing populations of human diploid fibroblasts was determined by 2-dimensional gel electrophoresis and [³²P]orthophosphate labelling. In quiescent (G₀) and G₁ cultures of HDF, lamin B₂ migrated as 2 spots on 2-dimensional gels. In contrast, in S-phase populations of HDF lamin B₂ migrated as a single basic species. The level of lamin B₂ phosphorylation was determined after immunoisolation from [³²P]orthophosphate labelled cells. The results of these experiments indicated a 2–3-fold increase in the steady state level of lamin B₂ phosphorylation in S-phase HDF compared with G₀ HDF. Consistent with this evidence, tryptic peptide maps revealed the presence of a phosphopeptide in S-phase lamin B₂ which was absent from G₀ lamin B₂. Since all of the phosphate incorporated into S-phase and G₀ lamin B₂ was recovered in serine residues we conclude that the S-phase specific phosphopeptide did not represent either of the cdc2 sites associated with entry nuclear lamina breakdown.

【 授权许可】

Unknown