Formation of 8‐nitroguanine in DNA treated with peroxynitrite in vitro and its rapid removal from DNA by depurination

FEBS Letters

Rubio, Julieta¹  Yermilov, Vladimir¹  Ohshima, Hiroshi¹ 

[1] Unit of Endogenous Cancer Risk Factors, International Agency for Research on Cancer, 150 Cours Albert Thomas, 69372 Lyon Cedex 08, France

关键词: Peroxynitrite;    8-Nitroguanine;    DNA damage;    8-Oxoguanine;    Nitric oxide;    Superoxide;    nitro8Gua;    8-nitroguanine;    oxo8Gua;    8-oxoguanine;    SIN-1;    3-(4-morpholinyl)-sydnone imine;    EC;    electrochemical detector;   

DOI  :  10.1016/0014-5793(95)01281-6

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Peroxynitrite is a strong oxidant formed by reaction of nitric oxide with superoxide in inflamed tissues. We have demonstrated that 8-nitroguanine is formed dose-dependently in calf thymus DNA incubated with low concentrations of peroxynitrite in vitro. 8-Nitroguanine in acid-hydrolyzed DNA was chemically reduced into 8-aminoguanine, which was analyzed using high performance liquid chromatography with electrochemical detection. Only peroxynitrite, but not nitrite, tetranitromethane nor NO-releasing compounds, formed 8-nitroguanine. Antioxidants and desferrioxamine inhibited the reaction. 8-Nitroguanine was depurinated from DNA incubated at pH 7.4, 37°C (). Peroxynitrite did not increase 8-oxoguanine levels in DNA.

【 授权许可】

Unknown