Effects of carbon dioxide/bicarbonate on induction of DNA single‐strand breaks and formation of 8‐nitroguanine, 8‐oxoguanine and base‐propenal mediated by peroxynitrite

FEBS Letters

Rubio, Julieta¹  Yermilov, Vladimir¹  Ohshima, Hiroshi¹  Yoshie, Yumiko¹ 

[1] Unit of Endogenous Cancer Risk Factors, International Agency for Research on Cancer, 150 Cours Albert Thomas, 69372 Lyon Cedex 08, France

关键词: Carbon dioxide;    Peroxynitrite;    8-Nitroguanine;    8-Oxoguanine;    DNA strand break;    Thymine-propenal;    nitro8Gua;    8-nitroguanine;    oxo8Gua;    8-oxoguanine;    amino8Gua;    8-aminoguanine;    EC;    electrochemical detector;    DTPA;    diethylenetriaminepentaacetic acid;    DMSO;    dimethylsulfoxide;   

DOI  :  10.1016/S0014-5793(96)01288-4

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Carbon dioxide has been reported to react with peroxynitrite (ONOO⁻), a strong oxidant and nitrating agent, to form an ONO₂CO₂ ⁻ adduct, altering the reactivity characteristic of peroxynitrite. We found that bicarbonate (0–10 mM) caused a dose-dependent increase of up to 6-fold in the formation of 8-nitroguanine in calf-thymus DNA incubated with 0.1 mM peroxynitrite, whereas it produced no apparent effect on 8-oxoguanine formation. In contrast, bicarbonate inhibited peroxynitrite-induced strand breakage in plasmid pBR322 DNA and thymine-propenal formation from thymidine. We conclude that reacts with peroxynitrite to form a potent nitrating agent, but also to inactivate hydroxyl-radical-like activity of peroxynitrous acid.

【 授权许可】

Unknown