Identification of the pore forming element of Semliki Forest virus spikes

FEBS Letters

Kempf, Christoph¹  Käsermann, Fabian¹  Spyr, Christian A.¹ 

[1] Institute of Biochemistry, University of Bern, Freiestrasse 3, 3012 Bern, Switzerland

关键词: Semliki Forest virus;    Pore formation;    E1 protein;   

DOI  :  10.1016/0014-5793(95)01197-M

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Pore formation at mildly acidic pH by SFV spike proteins was investigated using isolated and modified virions. Modification of the virions was performed by limited proteolysis in presence of octylglucoside and resulted in the formation of E1 particles and spikeless particles, respectively. Pore formation was detected by measuring the influx of propidium iodide into the viral particles. The results obtained clearly showed that the presence of E1 alone is sufficient to promote pore formation at mildly acidic pH. Thus E1 represents the pore forming element of the viral spike proteins.

【 授权许可】

Unknown