Expression of Semliki Forest virus capsid protein from SV40 recombinant virus

FEBS Letters

Jalanko, Anu¹ 

[1] Recombinant DNA Laboratory, University of Helsinki, Valimotie 7, SF-00380 Helsinki 38, Finland

关键词: Semliki Forest virus;    SFV capsid;    SV40 recombinant;    Proteolytic processing;    Expression;   

DOI  :  10.1016/0014-5793(85)81339-9

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Here, the proteolytic processing of the Semliki Forest virus (SFV) capsid protein was studied in the absence of other viral functions. Two different fragments of the SFV messenger cDNA, coding for capsid protein and 174 and 38 extra amino acids from the envelope proteins, respectively, were cloned in the late region of the SV40 viral DNA. Cells infected with the SV40 recombinant virus stocks were analyzed for the production of SFV capsid mRNA and polypeptide. Immunofluorescence staining of the infected cells indicated that the produced SFV capsid protein accumulated mainly in the nucleus. Polyacrylamide gel electrophoresis of the immunoprecipitated SFV capsid proteins showed that both recombinants yielded a labelled band equivalent in size to the SFV capsid protein. Thus the proteolytic processing takes place even under conditions where the capsid protein is the only virus-specified protein synthesized.

【 授权许可】

Unknown