Inverse deuterium kinetic isotope effect for peroxidation in human low‐density lipoprotein (LDL): a simple test for tocopherol‐mediated peroxidation of LDL lipids

FEBS Letters

Bowry, Vincent W.¹  Witting, Paul K.¹  Stocker, Roland¹ 

[1] Biochemistry Group, The Heart Research Institute, 145 Missenden Road, Camperdown, Sydney, N.S.W. 2050, Australia

关键词: Antioxidant;    Atherosclerosis;    Oxidation;    Vitamin E;   

DOI  :  10.1016/0014-5793(95)01172-B

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

α-Tocopherol (α-TOH) can act as a pro- or anti-oxidant for isolated ubiquinol-10-free human low density lipoprotein (LDL). We demonstrate that α-TOH is a more potent pro-oxidant than other forms of vitamin E for LDL peroxidation initiated by mild fluxes of aqueous peroxyl radicals and low concentrations of Cu²⁺. A simple deuterium exchange test shows that α-TOH switches from pro- to anti-oxidant at Cu²⁺: LDL ratios 2.5. The results suggest that this test may be useful to distinguish ‘inhibited’ perioxidation of emulsion lipids propagated via the lipid peroxyl radical from that mediated via the antioxidant radical.

【 授权许可】

Unknown