Ozone depletes tocopherols and tocotrienols topically applied to murine skin

FEBS Letters

Packer, Lester¹  Podda, Maurizio¹  Cross, Carroll E²  Traber, Maret G¹  Tsang, Kenneth¹  Thiele, Jens J¹ 

[1] Department of Molecular and Cell Biology, 251 LSA, University of California, Berkeley, CA 94720-3200, USA;Department of Medicine and Physiology, University of California School of Medicine, Davis, CA, USA

关键词: Hairless mice;    Vitamin E;    Antioxidant;    Malondialdehyde;    Epidermis;    Cutaneous lipid;    BHT;    butylated hydroxytoluene;    EDTA;    ethylene diamine tetraacetic acid;    HPLC-EC;    high pressure liquid chromatography with electrochemical detection;    PEG;    polyethylene glycol-400;    TRF;    tocotrienol-rich palm oil fraction;   

DOI  :  10.1016/S0014-5793(96)01463-9

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

To evaluate ozone damage to hairless mouse skin, two parameters of oxidative damage, vitamin E depletion and malondialdehyde (MDA) production, were measured in vitamin E-enriched and in control skin from mice exposed to ozone (10 ppm). A 5% vitamin E solution (tocotrienol-rich fraction, TRF) in polyethylene glycol (PEG) was applied to 2 sites on the back of hairless mice, PEG to 2 sites. After 2 h, the sites were washed, one of each pair of sites covered and the mice exposed ozone for 2 h. Ozone exposure (compared with covered sites) increased epidermal MDA in PEG-treated sites, while vitamin E was unchanged. In contrast, ozone exposure significantly depleted vitamin E in TRF-treated sites, while significant MDA accumulation was prevented. This is the first demonstration that ozone exposure causes damage to cutaneous lipids, an effect which can be attenuated by vitamin E application.

【 授权许可】

Unknown