IL‐2‐induced gene expression of protein‐tyrosine phosphatase LC‐PTP requires acidic and serine‐rich regions within IL‐2 receptor β chain

FEBS Letters

Taniguchi, Tadatsugu⁴  Yachi, Akira¹  Torigoe, Toshihiko³  Minami, Yasuhiro⁴  Sekiya, Masuo¹  Adachi, Masaaki¹  Hinoda, Yuji¹  Imai, Kohzoh¹  Reed, John C.² 

[1] First Department of Internal Medicine, Sapporo Medical University School of Medicine, Sapporo 060, Japan;La Jolla Cancer Research Foundation, 10901 N. Torrey Pine Road, La Jolla, CA 92037, USA;Department of Pathology, Sapporo Medical University School of Medicine, Sapporo 060, Japan;Institute for Molecular and Cellular Biology, Osaka University, Suita-shi, Osaka 565, Japan

关键词: Protein-tyrosine phosphatase;    LC-PTP;    IL-2;    Signal transduction;    PTK;    protein-tyrosine kinase;    PTP;    protein-tyrosine phosphatase;    LC-PTP;    leukocyte PTP;    IL-2;    interleukin-2;   

DOI  :  10.1016/0014-5793(95)00952-6

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

A protein-tyrosine phosphatase LC-PTP is preferentially expressed in hematopoietic cells and is an early response gene in lymphokine stimulated cells. Here, we found the LC-PTP mRNA induction by IL-2 was markedly inhibited by several tyrosine kinase inhibitors. The induction required both the acidic and serine-rich regions of the IL-2 receptor β chain (IL-2Rβ) in mouse IL-3-dependent pro-B BAF-B03 transfectants. This is strikingly different from the induction of c-myc gene expression, which requires the serine-rich region alone. In addition, overexpression of activated-Lck or -Raf kinases resulted in augmented LC-PTP mRNA expression in myeloid cell line 32D transfectants. Considering the previous findings that the acidic region of the IL-2Rβ is responsible for association with Lck and activation of Raf kinase, IL-2-induced expression of LC-PTP mRNA may be primarily transduced through a Lck-Raf mediated signaling pathway.

【 授权许可】

Unknown