Simulated microgravity inhibits the genetic expression of interleukin‐2 and its receptor in mitogen‐activated T lymphocytes

FEBS Letters

Cogoli, Augusto²  Walther, Isabelle²  Meloni, Maria Antonia¹  Mannu, Franca¹  Turrini, Franco¹  Pippia, Proto¹ 

[1]Department of Physiological, Biochemical and Cellular Sciences, University of Sassari, via Muroni 25, 17100 Sassari, Italy

[2]Space Biology, ETH Zurich, Technoparkstrasse 1, 8005 Zurich, Switzerland

关键词: T lymphocyte;    Gravitational biology;    Signal transduction;    Interleukin-2;    Interleukin-2 receptor;    Con A;    concanavalin A;    FRC;    fast rotating clinostat;    IL-1;    interleukin-1;    IL-2;    interleukin-2;    IL-2R;    interleukin-2 receptor;    PBL;    peripheral blood leukocytes;    PKC;    protein kinase C;    RPM;    Random positioning machine;    rpm;    rotation per minute;    RT-PCR;    reverse transcriptase-polymerase chain reaction;   

DOI  :  10.1016/S0014-5793(98)01107-7

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Experiments conducted in space in the last two decades have shown that T lymphocyte activation in vitro is remarkably reduced in microgravity. The data indicate that a failure of the expression of the interleukin-2 receptor (measured as protein secreted in the supernatant) is responsible of the loss of activity. To test such hypothesis we have studied the genetic expression of interleukin-2 and of its receptor in concanavalin A-activated lymphocytes with the RT-PCR technology. Microgravity conditions were simulated in the fast rotating clinostat and in the random positioning machine. The latter is an instrument introduced recently to study gravitational effects on single cells. Our data clearly show that the expression of both IL-2 and IL-2Rα genes is significantly inhibited in simulated 0×g. Thus full activation is prevented.

【 授权许可】

Unknown