Interleukin 2‐induced activation of JAK3: Possible involvement in signal transduction for c‐myc induction and cell proliferation

FEBS Letters

Tanaka, Nobuyuki¹  Nakamura, Masataka¹  Takeshita, Toshikazu¹  Sugamura, Kazuo¹  Higuchi, Masaya¹  Ishii, Naoto¹  Shirasawa, Takuji²  Asao, Hironobu¹ 

[1] Department of Microbiology, Tohoku University School of Medicine, Sendai 980-77, Japan;Department of Molecular Pathology, Tokyo Metropolitan Institute of Gerontology, 35-2 Sakae-cho, Itabashi-ku, Tokyo 173, Japan

关键词: IL-2;    IL-2Rγ;    JAK3;    Signal transduction;    IL-2;    interleukin 2;    IL-2R;    interleukin-2 receptor;    mAb;    monoclonal antibody;    SH2;    src homology region 2;   

DOI  :  10.1016/0014-5793(94)00831-0

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

We have investigated the role of JAK3 in interleukin 2 (IL-2)-induced signal transduction with a human T cell line, ED40515(−), lacking expression of the IL-2 receptor γ chain and its sublines transfected with wild-type or mutant cDNAs of the IL-2 receptor γ chain. Our results demonstrated that the membrane-proximal cytoplasmic region, encompassing the src homology region 2 (SH2)-like subdomain, of the γ chain is essential for association and activation of JAK3. Furthermore, IL-2-induced activation of JAK3 paralleled induction of the c-myc gene and DNA synthesis but not induction of the c-fos and c-jun genes. These results support the hypothesis that JAK3 plays a pivotal role in the IL-2 receptor-mediated signals for cell growth.

【 授权许可】

Unknown