Plasma 8‐epi‐PGF2α levels are elevated in individuals with non‐insulin dependent diabetes mellitus

FEBS Letters

Wolff, S.P.¹  Betteridge, D.J.¹  Gopaul, N.K.³  Änggård, E.E.³  Nourooz-Zadeh, J.¹  Mallet, A.I.² 

[1] Department of Medicine, Division of Clinical Pharmacology and Toxicology, University College London, 5 University Street, London WC1E 6JJ UK;St. John's Institute of Dermatology, Guy's and St. Thomas's Medical and Dental School, Lambeth Palace Road, London SE1 7EH, UK;The William Harvey Research Institute, St. Bartholomew's Hospital Medical College, Charterhouse Square, London EC1M 6BQ, UK

关键词: Prostaglandin;    F2-isoprostane;    Lipid peroxidation;    8-Epi-PGF2α;    Diabetes;    SPE;    solid-phase extraction;    GC-MS/NICI;    gas chromatography-mass spectrometry/negative ion chemical ionisation;    PFB;    pentafluorobenzyl;    TMS;    trimethylsilyl;    BSTFA;    N;    O-bis(trimethyl)-trifluoroacetamide;    BHT;    butylated hydroxytoluene;    DIPEA;    diisopropylethylamine;    NIDDM;    non-insulin-dependent diabetes mellitus;   

DOI  :  10.1016/0014-5793(95)00649-T

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

This study reports plasma levels of a specific nonenzymatic peroxidation product of arachidonic acid, esterified 8-epi-PGF_2α, from healthy- and NIDDM individuals as an index of oxidative stress in vivo. Plasma 8-epi-PGF_2α was isolated by solid-phase extraction on a C,8 followed by an NH₂ cartridge and analyzed by GC-MS/NICI as PFB-ester/TMS-ether derivative. We found that the average concentration of esterified 8-epi-PGF_2α among NIDDM subjects (0.93 ± 0.07 nM, n = 39) was higher (P < 0.0001, Mann-Whitney test) than in healthy individuals (0.28 ± 0.04 nM, n = 15). These data indicate that NIDDM is associated with increased plasma lipid peroxidation.

【 授权许可】

Unknown