Topological properties of two cubic phases of a phospholipid : cholesterol: diacylglycerol aqueous system and their possible implications in the phospholipase C‐induced liposome fusion

FEBS Letters

Nieva, J.L.²  Basáñez, G.²  Vargas, R.¹  Alonso, A.²  Luzzati, V.¹  Gulik, A.¹  Goñi, F.M.² 

[1] Centre de Génétique Moléculaire, C.N.R.S., 91198 Gif-sur-Yvette, France;Departamento de Bioquímica, Universidad del País Vasco, Aptdo. 644, 48080 Bilbao, Spain

关键词: Cubic phase;    Non-lamellar phase;    Phospholipid;    Diacylglycerol;    Membrane fusion;    CHOL;    cholesterol;    MG;    monoacylglycerol(s);    DG;    diacylglycerol(s);    PC;    phosphatidylcholine;    PE;    phosphatidyl-ethanolamine;    FA;    fatty acid;    PL;    phospholipids;   

DOI  :  10.1016/0014-5793(95)00631-I

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Water dispersions of phospholipid: cholesterol: diacylglycerol may, under certain conditions, originate either the lipid- and water-permeable Q²²⁴ cubic phase, or the lipid-permeable but water-impermeable Q²²⁷ cubic phase. These results are discussed within the framework of the phospholipase C-induced fusion of liposomes [Nieva et al. (1993) Biochemistry 32, 1054]. It is suggested that the cubic phases Q²²⁴ and Q²²⁷ represnt two classes of lipid organization, one promoting, the other hindering the mixing of aqueous contents that is characteristic of membrane fusion. In this context, inverted micelles appear to be the end point of the fusion process, rather than fusion intermediates.

【 授权许可】

Unknown