Orientational behavior of phospholipid membranes with mastoparan studied by 31P solid state NMR

FEBS Letters

Niidome, Takuro²  Aoyagi, Haruhiko²  Hori, Yumiko³  Asakura, Tetsuo³  Demura, Makoto¹ 

[1] Division of Biological Sciences, Graduate School of Science, Hokkaido University, Sapporo 060-0810, Japan;Faculty of Engineering, Nagasaki University, 1-14 Bunkyo-machi, Nagasaki 852-8521, Japan;Department of Biotechnology, Tokyo University of Agriculture and Technology, Koganei, Tokyo 184-8588, Japan

关键词: Mastoparan;    Nuclear magnetic resonance;    Membrane orientation;    Phospholipid;    Non-lamellar phase;   

DOI  :  10.1016/S0014-5793(99)00881-9

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Solid state ³¹P NMR spectroscopy was used to study the perturbing effect of the wasp venom peptide mastoparan (MP) on lipid bilayers composed of dimyristoylphosphatidylcholine (DMPC) and dimyristoylphosphatidylglycerol (DMPG). The ³¹P chemical shift anisotropy of multilamellar vesicles decreased with increasing peptide concentration, indicating that MP interacts strongly and selectively with the charged DMPG head group. Macroscopically oriented MP-lipid samples between glass plates were studied by ³¹P NMR as a function of tilt angle. These spectra showed the coexistence of orientation-dependent lamellar signals as well as an isotropic peak, suggesting that MP can induce non-lamellar phases in DMPC/DMPG membranes.

【 授权许可】

Unknown