Integrated lipidomics and RNA sequencing analysis reveal novel changes during 3T3-L1 cell adipogenesis

PeerJ

article

Yangli Pei¹  Yuxin Song¹  Bingyuan Wang²  Chenghong Lin¹  Ying Yang¹  Hua Li¹  Zheng Feng¹ 

[1] Guangdong Provincial Key Laboratory of Animal Molecular Design and Precise Breeding, School of Life Science and Engineering, Foshan University;Institute of Animal Sciences, Chinese Academy of Agricultural Sciences

关键词: Adipogenesis;    Lipidomics;    3T3-L1;    Triacylglycerol;    Diacylglycerol;    Sphingomyelin;    Ceramide;    Phospholipid;   

DOI  :  10.7717/peerj.13417

学科分类：社会科学、人文和艺术（综合）

来源: Inra

【 摘 要 】

After adipogenic differentiation, key regulators of adipogenesis are stimulated and cells begin to accumulate lipids. To identify specific changes in lipid composition and gene expression patterns during 3T3-L1 cell adipogenesis, we carried out lipidomics and RNA sequencing analysis of undifferentiated and differentiated 3T3-L1 cells. The analysis revealed significant changes in lipid content and gene expression patterns during adipogenesis. Slc2a4 was up-regulated, which may enhance glucose transport; Gpat3, Agpat2, Lipin1 and Dgat were also up-regulated, potentially to enrich intracellular triacylglycerol (TG). Increased expression levels of Pnpla2, Lipe, Acsl1 and Lpl likely increase intracellular free fatty acids, which can then be used for subsequent synthesis of other lipids, such as sphingomyelin (SM) and ceramide (Cer). Enriched intracellular diacylglycerol (DG) can also provide more raw materials for the synthesis of phosphatidylinositol (PI), phosphatidylcholine (PC), phosphatidylethanolamine (PE), ether-PE, and ether-PC, whereas high expression of Pla3 may enhance the formation of lysophophatidylcholine (LPC) and lysophosphatidylethanolamine (LPE). Therefore, in the process of adipogenesis of 3T3-L1 cells, a series of genes are activated, resulting in large changes in the contents of various lipid metabolites in the cells, especially TG, DG, SM, Cer, PI, PC, PE, etherPE, etherPC, LPC and LPE. These findings provide a theoretical basis for our understanding the pathophysiology of obesity.

【 授权许可】

CC BY