FEBS Letters | |
High expression of a novel carnitine palmitoyltransferase I like protein in rat brown adipose tissue and heart: isolation and characterization of its cDNA clone | |
Shinohara, Yasuo1  Terada, Hiroshi1  Shima, Atsushi1  Yamazaki, Naoshi1  | |
[1] Faculty of Pharmaceutical Sciences, University of Tokushima, Shomachi, Tokushima 770, Japan | |
关键词: Brown adipose tissue; Differential screening; cDNA cloning; Mitochondrion; Carnitine palmitoyltransferase; Rat; BAT; brown adipose tissue; WAT; white adipose tissue; UCP; uncoupling protein; CPTI; carnitine palmitoyltransferase I; CPTII; carnitine palmitoyltransferase II; PCR; polymerase chain reaction; bp; base pair(s); | |
DOI : 10.1016/0014-5793(95)00277-G | |
学科分类:生物化学/生物物理 | |
来源: John Wiley & Sons Ltd. | |
【 摘 要 】
To characterize energy metabolism in rat brown adipose tissue (BAT), we carried out differential screening of a cDNA library of BAT with a cDNA probe of white adipose tissue (WAT) and isolated one cDNA clone. It contained a single open reading frame of 2,316 bases which encodes a protein of 88.2 kDa. The predicted amino acid sequence showed the highest homology (62.6%) with that of rat carnitine palmitoyltransferase I (CPTI). The transcript corresponding to this cDNA was found to be abundantly expressed in BAT and heart. Therefore, the isolated clone is concluded to encode a CPTI like protein expressed in BAT and heart.
【 授权许可】
Unknown
【 预 览 】
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RO201912020300944ZK.pdf | 670KB | download |