High expression of a novel carnitine palmitoyltransferase I like protein in rat brown adipose tissue and heart: isolation and characterization of its cDNA clone

FEBS Letters

Shinohara, Yasuo¹  Terada, Hiroshi¹  Shima, Atsushi¹  Yamazaki, Naoshi¹ 

[1] Faculty of Pharmaceutical Sciences, University of Tokushima, Shomachi, Tokushima 770, Japan

关键词: Brown adipose tissue;    Differential screening;    cDNA cloning;    Mitochondrion;    Carnitine palmitoyltransferase;    Rat;    BAT;    brown adipose tissue;    WAT;    white adipose tissue;    UCP;    uncoupling protein;    CPTI;    carnitine palmitoyltransferase I;    CPTII;    carnitine palmitoyltransferase II;    PCR;    polymerase chain reaction;    bp;    base pair(s);   

DOI  :  10.1016/0014-5793(95)00277-G

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

To characterize energy metabolism in rat brown adipose tissue (BAT), we carried out differential screening of a cDNA library of BAT with a cDNA probe of white adipose tissue (WAT) and isolated one cDNA clone. It contained a single open reading frame of 2,316 bases which encodes a protein of 88.2 kDa. The predicted amino acid sequence showed the highest homology (62.6%) with that of rat carnitine palmitoyltransferase I (CPTI). The transcript corresponding to this cDNA was found to be abundantly expressed in BAT and heart. Therefore, the isolated clone is concluded to encode a CPTI like protein expressed in BAT and heart.

【 授权许可】

Unknown