FEBS Letters | |
Transcriptional regulation of nuclear orphan receptor, NOR‐1, by Ca2+/calmodulin‐dependent protein kinase cascade | |
Kobayashi, Ryoji1  Inuzuka, Hiroyuki1  Tokumitsu, Hiroshi1  Ohkura, Naganari2  | |
[1] Department of Chemistry, Kagawa Medical University, 1750-1 Miki-cho, Kita-gun, Kagawa 761-0793, Japan;National Cancer Institute Research Center, 5-1-1 Tsukiji, Tokyo 104-0045, Japan | |
关键词: Ca2+; Ca2+/calmodulin-dependent protein kinase IV cascade; Neuron-derived orphan receptor-1; Ca2+/calmodulin-dependent protein kinase kinase; Differential screening; NOR-1; neuron-derived orphan receptor-1; CaM-K; Ca2+/calmodulin-dependent protein kinase; CRE; cAMP response element; | |
DOI : 10.1016/S0014-5793(02)02890-9 | |
学科分类:生物化学/生物物理 | |
来源: John Wiley & Sons Ltd. | |
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【 摘 要 】
We found that transcription of NOR-1 (neuron-derived orphan receptor-1) is induced by the Ca2+/calmodulin-dependent protein kinase IV (CaM-KIV) cascade by differential hybridization screening of a cDNA array using probes prepared from SH-SY5Y cells infected with recombinant adenoviruses expressing Ca2+/CaM-independent mutants of CaM-KIV and CaM-K kinase (CaM-KK). Induction of NOR-1 mRNA expression by the CaM-KIV cascade was confirmed by RT-PCR. Promoter analyses indicate that CaM-KIV cascade response element is located between −162 bp and −42 bp in the 1.7 kb NOR-1 promoter containing triple cAMP response elements. Disruption of each element significantly reduced the promoter activity, indicating the direct transcriptional regulation of NOR-1 by CaM-KIV cascade.
【 授权许可】
Unknown
【 预 览 】
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RO201912020311942ZK.pdf | 204KB | ![]() |