Transcriptional regulation of nuclear orphan receptor, NOR‐1, by Ca2+/calmodulin‐dependent protein kinase cascade

FEBS Letters

Kobayashi, Ryoji¹  Inuzuka, Hiroyuki¹  Tokumitsu, Hiroshi¹  Ohkura, Naganari² 

[1] Department of Chemistry, Kagawa Medical University, 1750-1 Miki-cho, Kita-gun, Kagawa 761-0793, Japan;National Cancer Institute Research Center, 5-1-1 Tsukiji, Tokyo 104-0045, Japan

关键词: Ca2+;    Ca2+/calmodulin-dependent protein kinase IV cascade;    Neuron-derived orphan receptor-1;    Ca2+/calmodulin-dependent protein kinase kinase;    Differential screening;    NOR-1;    neuron-derived orphan receptor-1;    CaM-K;    Ca2+/calmodulin-dependent protein kinase;    CRE;    cAMP response element;   

DOI  :  10.1016/S0014-5793(02)02890-9

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

We found that transcription of NOR-1 (neuron-derived orphan receptor-1) is induced by the Ca²⁺/calmodulin-dependent protein kinase IV (CaM-KIV) cascade by differential hybridization screening of a cDNA array using probes prepared from SH-SY5Y cells infected with recombinant adenoviruses expressing Ca²⁺/CaM-independent mutants of CaM-KIV and CaM-K kinase (CaM-KK). Induction of NOR-1 mRNA expression by the CaM-KIV cascade was confirmed by RT-PCR. Promoter analyses indicate that CaM-KIV cascade response element is located between −162 bp and −42 bp in the 1.7 kb NOR-1 promoter containing triple cAMP response elements. Disruption of each element significantly reduced the promoter activity, indicating the direct transcriptional regulation of NOR-1 by CaM-KIV cascade.

【 授权许可】

Unknown