Low content of mitochondrial ATPase in brown adipose tissue is the result of post‐transcriptional regulation

FEBS Letters

Baudyšová, Marie¹  Tvrdík, Peter¹  Pavelka, Stanislav¹  Houštěk, Josef¹ 

[1] Institute of Physiology, Czechoslovak Academy of Sciences, 142 20 Prague, Czechoslovakia

关键词: Brown adipose tissue;    Mitochondrion;    ATPase;    Cytochrome oxidase;    Transcription;    Nuclear gene;    BAT;    brown adipose tissue;    ATPase;    mitochondrial ATP synthase;    F0;    membrane part of ATPase;    F1;    catalytic part of ATPase;    UCP;    uncoupling protein;    COX;    cytochrome c oxidase;   

DOI  :  10.1016/0014-5793(91)80666-Q

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The mRNA levels of ATPase β, ATPase 6, cytochrome oxidase (COX) VIb and COX I subunits were found to be 2.4–13.8-fold higher in brown adipose tissue (BAT) than in heart, skeletal muscle, brain and liver of mice. The comparison with tissue contents of ATPase and COX revealed that the selective, 5–11-fold reduction of ATPase in BAT is not caused by decreased transcription of ATPase genes. Likewise, the ATPase β and COX VIb mRNA levels in cultured brown adipocytes were also not influenced by norepinephrine, which activated the expression of the UCP gene by two orders of magnitude. The results indicate that the biosynthesis of mitochondrial ATPase in BAT is post-transcriptionally regulated.

【 授权许可】

Unknown