FEBS Letters | |
Low translational efficiency of the F1‐ATPase β‐subunit mRNA largely accounts for the decreased ATPase content in brown adipose tissue mitochondria | |
Kužela, Štefan1  Tvrdik, Petr2  Houštĕk, Josef2  | |
[1] Institute of Molecular Biology, Slovak Academy of Sciences, 842 51 Bratislava, Czechoslovakia;Institute of Physiology, Czechoslovak Academy of Sciences, 142 20 Prague, Czechoslovakia | |
关键词: Brown adipose tissue; ATPase; mRNA half-life; Translational efficiency; BAT; brown adipose tissue; ATPase; mitochondrial ATP synthase; F1-β; β subunit of the catalytic F1-part of mitochondrial ATP synthase; UCP; uncoupling protein; | |
DOI : 10.1016/0014-5793(92)81175-L | |
学科分类:生物化学/生物物理 | |
来源: John Wiley & Sons Ltd. | |
【 摘 要 】
The half-life of the F1-ATPase β-subunit (F1-β) mRNA in ATPase-poor brown adipose tissue (BAT) (t?? = 9.5 h) was found to be 3–7-fold shorter than in liver (t?? = 27 h) and heart (t?? = 63 h) of mice. When translated in reticulocyte lysate, a 2–3-fold lower efficiency appeared with F1-β mRNA from BAT than from other tissues. The in vitro synthesized F1-β protein precursors of BAT, liver and heart origin were imported and processed by mouse liver mitochondria with equal efficiency. The results indicate that the pool or abundant F1-β mRNA in BAT is not fully translatable most likely due to its low metabolic stability.
【 授权许可】
Unknown
【 预 览 】
Files | Size | Format | View |
---|---|---|---|
RO201912020297087ZK.pdf | 314KB | download |