Low translational efficiency of the F1‐ATPase β‐subunit mRNA largely accounts for the decreased ATPase content in brown adipose tissue mitochondria

FEBS Letters

Kužela, Štefan¹  Tvrdik, Petr²  Houštĕk, Josef² 

[1] Institute of Molecular Biology, Slovak Academy of Sciences, 842 51 Bratislava, Czechoslovakia;Institute of Physiology, Czechoslovak Academy of Sciences, 142 20 Prague, Czechoslovakia

关键词: Brown adipose tissue;    ATPase;    mRNA half-life;    Translational efficiency;    BAT;    brown adipose tissue;    ATPase;    mitochondrial ATP synthase;    F1-β;    β subunit of the catalytic F1-part of mitochondrial ATP synthase;    UCP;    uncoupling protein;   

DOI  :  10.1016/0014-5793(92)81175-L

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The half-life of the F₁-ATPase β-subunit (F₁-β) mRNA in ATPase-poor brown adipose tissue (BAT) (t?? = 9.5 h) was found to be 3–7-fold shorter than in liver (t?? = 27 h) and heart (t?? = 63 h) of mice. When translated in reticulocyte lysate, a 2–3-fold lower efficiency appeared with F₁-β mRNA from BAT than from other tissues. The in vitro synthesized F₁-β protein precursors of BAT, liver and heart origin were imported and processed by mouse liver mitochondria with equal efficiency. The results indicate that the pool or abundant F₁-β mRNA in BAT is not fully translatable most likely due to its low metabolic stability.

【 授权许可】

Unknown