Eukaryotic mRNAs encoding abundant and scarce proteins are statistically dissimilar in many structural features

FEBS Letters

Kolchanov, Nikolay A²  Kel, Alexander E²  Babenko, Vladimir N²  Kochetov, Alex V²  Ischenko, Igor V²  Kisselev, Lev L¹  Vorobiev, Denis G² 

[1] Engelhardt Institute of Molecular Biology, Moscow, 117984 Russia;Institute of Cytology and Genetics, Pr. Lavrentieva 10, Novosibirsk, 630090 Russia

关键词: Eukaryotic mRNA;    Translational efficiency;    5′ Untranslated region;    Context effect;    Statistical analysis;    Exp;    expected;    H-mRNA and L-mRNA;    eukaryotic mRNAs encoding highly abundant and scarce proteins;    respectively;    Obs;    observed;    UTR;    untranslated region of mRNA;   

DOI  :  10.1016/S0014-5793(98)01482-3

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

It is well known that non-coding mRNA sequences are dissimilar in many structural features. For individual mRNAs correlations were found for some of these features and their translational efficiency. However, no systematic statistical analysis was undertaken to relate protein abundance and structural characteristics of mRNA encoding the given protein. We have demonstrated that structural and contextual features of eukaryotic mRNAs encoding high- and low-abundant proteins differ in the 5′ untranslated regions (UTR). Statistically, 5′ UTRs of low-expression mRNAs are longer, their guanine plus cytosine content is higher, they have a less optimal context of the translation initiation codons of the main open reading frames and contain more frequently upstream AUG than 5′ UTRs of high-expression mRNAs. Apart from the differences in 5′ UTRs, high-expression mRNAs contain stronger termination signals. Structural features of low- and high-expression mRNAs are likely to contribute to the yield of their protein products.

【 授权许可】

Unknown