Inhibition of the DNA‐binding activity of NF‐κB by gold compounds in vitro

FEBS Letters

Merin, Jocelyn P.¹  Nakano, Tatsunori¹  Kitade, Yukio²  Yang, Jian-Ping¹  Okamoto, Takashi¹  Kato, Tetsuji¹ 

[1] Department of Molecular Genetics, Nagoya City University Medical School, 1 Kawasumi, Mizuho-cho, Mizuho-ku, Nagoya 467, Japan;Department of Chemistry, Faculty of Engineering, Gifu University, 1-1 Yanagido, Gifu 501-11, Japan

关键词: NF-κB;    Gold;    Zinc;    DNA binding;    Rheumatoid arthritis;    NF-κB;    nuclear factor κB;    CAMs;    cell adhesion molecules;    RA;    rheumatoid arthritis;    oPA;    1;    10-orthophenanthroline;    DTT;    dithiothreitol;    AuTG;    aurothioglucose;    DPA;    d-penicillamine;    AuTM;    aurothiomalate;    EMSA;    electrophoretic mobility shift assay;   

DOI  :  10.1016/0014-5793(95)00157-5

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Nuclear factor κB (NF-κB) is a transcription factor that is critical for the inducible expression of multiple cellular and viral genes. DNA binding activity is essential for its function. Here, we report that gold compounds, especially aurothioglucose (AuTG), have a strong inhibitory effect on NF-κB-DNA binding. Our finding also reveals that Zn²⁺ is a necessary component of NF-κB for its DNA binding activity and that gold ion can efficiently block NF-κB-DNA binding, presumably through oxidation of the cysteins associated with zinc. This redox mechanism may provide an explanation for the observed efficacy of gold compounds in the treatment of rheumatoid arthritis.

【 授权许可】

Unknown