FEBS Letters | |
Zinc is required in pyrrolidine dithiocarbamate inhibition of NF‐κB activation | |
Kim, Chul Hoon2  Ahn, Young Soo2  Kim, Joo Hee2  Hsu, Chung Y.1  | |
[1] Department of Neurology, Washington University School of Medicine, St. Louis, MO 63110, USA;Yonsei Brain Research Institute and Department of Pharmacology, Yonsei University College of Medicine, Seoul 120-752, South Korea | |
关键词: Dithiocarbamate; NF-κB; Zinc; BCECs; bovine cerebral endothelial cells; EDTA; ethylenediaminetetraacetic acid; EMSA; electrophoretic mobility shift assay; FBS; fetal bovine serum; PDTC; pyrrolidine dithiocarbamate; NF-κB; nuclear factor kappa B; LDH; lactate dehydrogenase; | |
DOI : 10.1016/S0014-5793(99)00390-7 | |
学科分类:生物化学/生物物理 | |
来源: John Wiley & Sons Ltd. | |
【 摘 要 】
Pyrrolidine dithiocarbamate (PDTC) is a potent inhibitor of nuclear factor kappa B (NF-κB) activation. PDTC inhibited basal NF-κB activity of endothelial cells. PDTC, however, failed to inhibit basal NF-κB activity after withdrawal of serum in the media, and the inhibitory effect of PDTC could be restored by addition of zinc. When various preparations of metal ion-EDTA were tested with PDTC in serum-containing media, only Zn-EDTA failed to block the inhibitory effect of PDTC. The dependence on zinc was also noted in PDTC inhibition of NF-κB stimulated by TNFα. These facts suggest that zinc is required for PDTC inhibition of NF-κB activation.
【 授权许可】
Unknown
【 预 览 】
Files | Size | Format | View |
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RO201912020307566ZK.pdf | 207KB | download |