Zinc is required in pyrrolidine dithiocarbamate inhibition of NF‐κB activation

FEBS Letters

Kim, Chul Hoon²  Ahn, Young Soo²  Kim, Joo Hee²  Hsu, Chung Y.¹ 

[1] Department of Neurology, Washington University School of Medicine, St. Louis, MO 63110, USA;Yonsei Brain Research Institute and Department of Pharmacology, Yonsei University College of Medicine, Seoul 120-752, South Korea

关键词: Dithiocarbamate;    NF-κB;    Zinc;    BCECs;    bovine cerebral endothelial cells;    EDTA;    ethylenediaminetetraacetic acid;    EMSA;    electrophoretic mobility shift assay;    FBS;    fetal bovine serum;    PDTC;    pyrrolidine dithiocarbamate;    NF-κB;    nuclear factor kappa B;    LDH;    lactate dehydrogenase;   

DOI  :  10.1016/S0014-5793(99)00390-7

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Pyrrolidine dithiocarbamate (PDTC) is a potent inhibitor of nuclear factor kappa B (NF-κB) activation. PDTC inhibited basal NF-κB activity of endothelial cells. PDTC, however, failed to inhibit basal NF-κB activity after withdrawal of serum in the media, and the inhibitory effect of PDTC could be restored by addition of zinc. When various preparations of metal ion-EDTA were tested with PDTC in serum-containing media, only Zn-EDTA failed to block the inhibitory effect of PDTC. The dependence on zinc was also noted in PDTC inhibition of NF-κB stimulated by TNFα. These facts suggest that zinc is required for PDTC inhibition of NF-κB activation.

【 授权许可】

Unknown