Effects of lovastatin on adenylyl cyclase activity and G proteins in GH4C1 cells

FEBS Letters

Brihuega, Fuensanta²  Montes, Agustín¹  Lasa, Marina²  Chiloeches, Antonio²  Toro, María J.² 

[1] Department of Physiology and Pharmacology, University of Alcalá, Crta. Madrid-Barcelona Km. 33,600,28871 Alcalá de Henares, Madrid, Spain;Department of Biochemistry and Molecular Biology, University of Alcalá, Crta. Madrid-Barcelona Km. 33,6000,28871 Alcalá de Henaris, Madrid, Spain

关键词: G-protein;    Adenylyl cyclase;    Cholesterol;    Lovastatin;   

DOI  :  10.1016/0014-5793(95)00139-Z

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

We studied the effect of lovastatin, a cholesterol lowering drug, on the basal state of G-proteins in GH₄C₁ cells. Our data show that the addition of lovastatin markedly decreased the amount of the α-subunits of the G_s and G_i-proteins in the plasma membrane. The decrease of αs was correlated with a decrease in adenylyl cyclase activity, and both effects were reverted by the presence of mevalonate. As the attachment of G protein subunits to the membrane is dependent on γ-subunit prenylation, we assume that the mechanism through which lovastatin exerts its effects on G-proteins is the lack of mevalonate for the synthesis of prenyl residues. In conclusion, our data indicate that some of the effects of lovastatin are mediated through changes in the basal state of G-protein in the membrane and consequently on adenylyl cyclase activity.

【 授权许可】

Unknown