Enzymatic and immunological detection of G protein α‐subunits in the pathogenic fungus Candida albicans

FEBS Letters

Montero, Lilian¹  Passeron, Susana¹  Paveto, Cristina¹ 

[1] Departamento de Quimica Biológica, Facultad de Ciencias Exactas y Naturales, Universidad de Buinos Aires and CONICET, Ciudad Universitaria, Pabellón II, 40 Piso, Buenos Aires, Argentina

关键词: G-protein;    Peptide antiserum;    α-commons;    Adenylyl cyclase;    Hormone response;    Candida albicans;    PMSF;    phenyl-methylsulfonyl fluoride;    GTP-γ-S;    guanosine 5′-O-(3-thio-triphosphate);   

DOI  :  10.1016/0014-5793(92)81365-S

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

GTP stimulation of adenylyl cyclase from the dimorphic pathogenic fungus Candida albicans is greatly enhanced by preincubation of membrane proteins with cholera toxin, NAD and ATP. In the presence of [³²P]NAD the toxin catalyzes the covalent incorporation of radioactivity into a menbrane protein of 40 kDa. Pertussis toxin catalyzes the transference of the radioactivity from [³²P]NAD to a 32 kDa protein. Two major proteins of 40–42 and 30–32 kDa can also be recognized in Western blots by an anti G_α-common antibody. The results support the idea that G proteins are part of the hormonesensory transduction chain of Candida [(1990) Biochem. Biophys. Res. Commun. 167. 1177-1183].

【 授权许可】

Unknown