Regulation of adenylyl cyclase from Blastocladiella emersonii by guanine nucleotides

FEBS Letters

Terenzi, Hernán¹  da Costa Maia, José Carlos¹ 

[1] Departamento de Bioquimica, Instituto de Quimica, Universidade de São Paulo, Caixa Postal 20780, São Paulo, SP 01498-970, Brazil

关键词: G-protein;    Adenylyl cyclase;    Blastocladiella emersonii;    PMSF;    phenyl-methylsulfonyl fluoride;    GTPγS;    guanosine 5′-O-(3-thio-triphosphate);    GDPβS;    guanosine 5′-(O-2-thio)-diphosphate;    IBMX;    isobutyl-methyl-xanthine;   

DOI  :  10.1016/0014-5793(93)81669-Q

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

GTPγS stimulates adenylyl cyclase in particulate fractions of Blastocladiella emersonii zoospores. Cholera toxin catalyses the ADP-ribosylation of a membrane protein of a molecular weight (46,000) similar to that of the α subunit of G_s found in vertebrate cells. A membrane protein of 46 kDa can also be recognized in Western blots by an antipeptide antiserum (RM/1) raised against the C-terminus of Gα _s -subunits. These results suggest that a G-protein mediates the regulation of Blastocladiella adenylyl cyclase by guanine nucleotides.

【 授权许可】

Unknown