Molecular cloning and functional expression of a novel potassium channel β‐subunit from human atrium

FEBS Letters

De Biasi, Mariella¹  Wible, Barbara A.¹  Wang, Zhiguo¹  Majumder, Kumud¹ 

[1] Department of Molecular Physiology and Biophysics, Baylor College of Medicine, Houston, TX 77030, USA

关键词: Potassium channel;    β-Subunit;    Human heart;    cDNA cloning;    Xenopus oocyte;    SDS;    sodium dodecyl sulfate;    NMDG;    MES;    2(N-morpholino)ethanesulphonic acid;    HEPES;    N-2-hydroxyethyl-piperazine-N′-2-ethanesulfoni c acid;    SSC;    saline-sodium citrate;    PCR;    polymerase chain reaction;    RT-PCR;    reverse transcriptase polymerase chain reaction;    kb;    kilobase pairs;    bp;    base pairs;    N-terminal;    amino-terminal;   

DOI  :  10.1016/0014-5793(95)00120-X

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

We report the cloning and functional expression of a novel K⁺ channel β-subunit from human atrium, hKvβ3. hKvβ3 is highly homologous to the two β-subunits cloned from rat brain, Kvβ1 and Kvβ2, but has an essentially unique stretch of 79 N-terminal residues. Upon expression in Xenopus oocytes, hKvβ3 accelerates the inactivation of co-injected hKv1.4 currents and induces fast inactivation of non-inactivating co-injected hKv1.5 currents. By contrast, hKvβ3 had no effect on hKv1.1, hKv1.2, or hKv2.1 currents. Thus, hKvβ3 represents a third type of K⁺ channel β-subunit which modulates the kinetics of a unique subset of channels in the Kv1 subfamily.

【 授权许可】

Unknown