Cloning and tissue‐specific expression of the brain calcium channel β‐subunit

FEBS Letters

Jay, Scott D.¹  Pragnell, Marlon²  Campbell, Kevin P.³  Sakamoto, Junshi³ 

[1] Department of Physiology and Biophysics, University of Iowa College of Medicine, Iowa City, IA 52242, USA;Program in Neuroscience, University of Iowa College of Medicine, Iowa City, IA 52242, USA;Howard Hughes Medical Institute, University of Iowa College of Medicine, Iowa City, IA 52242, USA

关键词: β-Subunit;    Calcium channel;    cDNA cloning;    Brain;   

DOI  :  10.1016/0014-5793(91)81296-K

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

A cDNA clone encoding a protein with high homology to the β-subunit of the rabbit skeletal muscle dihydropyridine-sensitive calcium channel was isolated from a rat brain cDNA library. This rat brain β-subunit cDNA hybridizes to a 3.4 kb message that is expressed in high levels in the cerebral hemispheres and hippocampus but is significantly reduced in cerebellum. The open reading frame encodes 597 amino acids with a predicted mass of 65 679 Da which is 82% homologous with the skeletal muscle β-subunit. The brain cDNA encodes a unique 153 amino acid C-terminus and predicts the absence of a muscle-specific 50 amino acid internal segment. It also encodes numerous consensus phosphorylation sites suggesting a role in calcium channel regulation. The corresponding human β-subunit gene was localized to chromosome 17. Hence the encoded brain β-Subunit. which has a primary structure highly similar to its isoform in skeletal muscle, may have a comparable role as an integral regulatory component of a neuronal calcium channel.

【 授权许可】

Unknown